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Â鶹AV.CA / Rosalind and Morris Goodman Cancer Institute / Facilities

Â鶹AV Platform for Cellular Perturbation (MPCP)

The Â鶹AV Platform for Cell Perturbation (MPCP) provides all Â鶹AV researchers with low-cost and rapid access to the latest functional genetic tools for biological research. The MPCP was established in January 2017 and is part of the Rosalind and Morris Goodman Cancer Research Center (GCRC) and of the Biochemistry department. The mission of the MPCP is to support functional investigations of mammalian genomes by providing Â鶹AV researchers with complementary means of genetic perturbation that can be applied in order to understand gene function. These comprehensive technological platforms include arrayed and sequence-verified lentiviral genome-wide shRNA (gene suppression), cDNA (for gene overexpression) and CRISPR (for gene knockout/editing; human) libraries. These collections, in arrayed bacterial glycerol stocks, offer a renewable resource for diverse customized screening or testing of individual genes of interest across the human and mouse genomes.

Platform Director: Dr. Sidong Huang (sidong.huang [at] mcgill.ca)
Platform Manager:ÌýDr. Azadeh Arabzadeh (azadeh.arabzadeh [at] mcgill.ca)

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It is typically very costly and time-consuming to obtain these reagents from commercial vendors. Our core service provides Â鶹AV researchers with rapid access to these essential genetic tools with at a minimal fee, greatly enhancing their research capacities.

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The facility, located in the Huang laboratory on the 8th floor of the McIntyre Medical building, includes a dedicated molecular biology wet lab and general office as well as shared access to a bacterial culture room and freezer farm space of the Biochemistry department.

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List of genome-wide plasmid libraries:

MISSION® Human & Mouse lentiviral shRNA libraries (TRC1, 1.5 and 2)

MISSION® TRC3 Human ORF collection (cDNA)

MGC premier human lentiviral ORF, ORFeome Collaboration collection, ORFeome v8.1 Libraries

MGC premier mouse ORFeome Collaboration Library

transEDIT-dual CRISPR arrayed library, Human

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Main Instruments:

4x Ultra-Low Temperature Freezer, Panasonic model MDF-U76V

2x Infors HT Ecotron shaker incubator

1x VisionMate SR single rack 2D barcode reader

1x NanoDrop ND-2000C spectrophotometer

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